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Dr. Deborah Fuller Addresses the Omicron Variant, Vaccines and Travel Bans

WaNPRC AD for Research speaks to media about new SARS-CoV-2 variant of concern

WaNPRC Announces Appointment of New Director

After a national search for a new center director, the WaNPRC has been awarded with the recruitment of an internationally recognized outside candidate. Effective October 16, 2021, Michele A. Basso, PhD, will join the WaNPRC as the new director.

Dr. Basso is already discussing plans for recruitment of new investigators and bringing in new core staff, particularly in the field of HIV and at the intersection of neuroscience and infectious disease.

 

Kyes Stresses Importance of Monitoring Wildlife and Human Interactions in Nepal

The Natural Resource Management Program at Nepal College of Engineering (NEC), in association with University of Washington, organized an August 20 webinar entitled, “Bush to Bench to Bedside: The Critical Role of Field Research in Biodiversity Conservation and Translational Science.”

The keynote speaker at the webinar, Professor Randall C. Kyes, said that long-term monitoring is very important to find out the status of biodiversity. He presented his research experience in different countries and emphasized on possible zoonotic diseases passing between humans and wildlife. Speaking about research on methicillin-resistant Staphylococcus aureus (MRSA) in monkeys of Kathmandu Valley. The Master of Sciences in Natural Resources Management program at the Center for Postgraduate Studies (CPS) is a collaborative partner for this research. Kyes said that evidence of human-to-human transmission of MRSA bacteria has been found in monkeys of Kathmandu Valley.

Warning of the potential dangers to wildlife from human and wildlife interactions, he said there is a need for ongoing research on wildlife and biodiversity. The webinar was attended by graduate students from various colleges. NEC CPS regularly organizes a series of webinars on various topics of biodiversity conservation and natural resource management.

  • The above is a translation of an article from Nagarik News, a Nepali language daily newspaper and news network, Nepal Republic Media Pvt. Ltd.
  • Dr. Randall C. Kyes is chief of the Division of Global Programs at the Washington National Primate Research Center.

NIAID Now: The Important Role of Animal Research in mRNA COVID-19 Vaccine Development

As the global COVID-19 pandemic continues, safe and effective vaccines are playing a pivotal role in preventing severe disease and death and limiting the spread of SARS-CoV-2, the virus that causes COVID-19. The urgency of the COVID-19 pandemic necessitated rapid vaccine development and testing. Fortunately, NIAID’s decades-long support and conduct of coronavirus and vaccine research laid the groundwork for helping to develop a safe and effective COVID-19 vaccine in record speed.

COVID-19 Animal Models

Animal research plays a key role in developing successful vaccines for humans. Before promising vaccine candidates can be tested in humans, they must first be tested for safety and effectiveness in animals as required by the U.S. Food and Drug Administration. To do this, scientists first determine whether a vaccine candidate can stimulate an adequate and safe immune response. This important step is often conducted using small and then, potentially, larger animal models of disease. Mice are frequently used because they reproduce rapidly, have a well-characterized immune system and a defined genome. Some labs turned to mouse models of infection early in the COVID-19 pandemic only to find that mice don’t get infected with SARS-CoV-2. In order to infect cells, SARS-CoV-2 must bind to a human protein called ACE2. The human and mouse ACE2 proteins are different, and SARS-CoV-2 does not bind to mouse cells. Scientists overcame this problem by generating mice that can express the human version of ACE2 and can therefore be infected with SARS-CoV-2. When these genetically modified mice are infected by the virus, they lose weight and become sick in ways that are similar to what happens when people are infected with the virus. Mouse models provided vital information about COVID-19 symptoms and its disease course and continues to be used by researchers to understand COVID-19 disease.

Syrian hamsters are another important animal model for COVID-19 because disease in those animals closely resembles the disease in humans. Additionally, older male hamsters develop more severe disease than young female hamsters, which reflects some of the differences seen in humans infected by SARS-CoV-2. Hamster models have contributed to the evaluation of investigational COVID-19 vaccine candidates, immunotherapies, and antiviral drugs.

Vaccine development for COVID-19 also benefitted from nonhuman primate studies. In assessing immunogenicity and protection of vaccines in pre-clinical animal models, nonhuman primates provide several advantages for clinical translation. They are outbred, have greater similarity to humans than rodents in innate immune responses and B- and T-cell repertoires, and allow use of clinically-relevant vaccine doses. Recent studies in nonhuman primates show that SARS-CoV-2 targets similar replication sites and recapitulates some aspects of COVID-19 disease.  Nonhuman primates are used during the later stages of vaccine development and typically build upon the knowledge accumulated in earlier small animal studies.

Continue reading…

Retinal Researchers Tap Into TDP to Learn More About Predictive Coding

by Michael McCarthy

Professor Michael Manookin works at a confocal microscope.
Finding helps explain how baseball players can connect with a 100-mph fastball and how the rest of us manage everyday tasks.

Neural circuits in the primate retina can generate the information needed to predict the path of a moving object before visual signals even leave the eye, UW Medicine researchers demonstrate in a new paper.

“The ability to predict where moving objects will go is so important for survival that it’s likely hardwired into all sighted animals,” said Michael Manookin, an assistant professor of ophthalmology at the University of Washington School of Medicine. He led the research team with Fred Rieke, professor of physiology and biophysics.

Manookin and his colleagues report their findings in the journal Nature Neuroscience.  Belle Liu and Arthur Hong, two UW undergraduate students, were the lead authors on the paper.

In the study, the researchers looked at how motion was processed by cellular circuits in the retina. The circuits the researchers focused on are composed of light-sensing photoreceptor cells, called cones; an intermediate layer of cells, called bipolar cells; and ganglion cells that collect signals from bipolar cells and transmit these signals out of the eye to other brain regions.

Continue reading at UW Medicine Newsroom.

 

Gordon: Got a COVID vaccination? You can thank research and science

Ken Gordon
Ken Gordon, executive director of the Northwest Association of Biomedical Research (NWABR)

Just like our doctors and nurses, researchers in the Pacific Northwest have also been on the frontlines working at a rapid pace to understand the novel coronavirus that causes COVID-19: how it spreads, how it infects, who it infects, why it shows itself in a variety of symptoms and why it progresses in such a deadly way for some people.

Now more than 175 million Americans, and nearly 70% of eligible Oregonians, are directly benefiting from the biomedical research that developed these vaccines, bringing an end in sight to the pandemic.

Sadly, some animal rights organizations have used misinformation and fake news to disparage the biomedical research process during the past year.

Before anyone can be treated or vaccinated in America, medicines go through a lengthy process of lab research, humane animal modeling and human studies to ensure they work and are safe.

For the coronavirus vaccines this work started in libraries as researchers scoured the literature on coronaviruses and looked at decades of prior animal and human studies on vaccines. With this information in hand, researchers moved to their laboratories, where they identified ways to stimulate immunity to COVID-19.

The next step involved humane animal modeling to see if lab-developed products worked in living organisms. These animal studies typically started with mice, and if the results looked good then they proceed to working with animals like monkeys, which share more similarities with humans.

The last step involved human studies that started initially with just a few people and moved to trials that involved tens of thousands of volunteers. These studies were aimed at determining both the safety and the effectiveness of these vaccines.

We are all incredibly lucky researchers were able to develop three (with more on the way) highly effective COVID-19 vaccines in such a short time. Vaccine development work is still progressing with researchers at the Oregon Health and Science University working on the next generation of vaccines for COVID-19.

I understand how all this work created an existential crisis for animal rights groups, who oppose the use of any animals in research. But that’s no excuse to spin twisted stories about research and intimidate researchers.

Millions of Americans have directly benefited from ethical biomedical research and are now protected from severe symptoms and hospitalization due to COVID-19. Without animal studies we would not have these vaccines and we would still be in the thick of one of the worst pandemics in history.

Even worse, an animal rights supporter at a local university recently said he would prefer to have millions of people die if it meant an end to animal studies. Another anti-research activist compared animal researchers to child traffickers.

The people who are suffering the most from COVID-19 are people of color, people with underlying health conditions, people who are older and people with disabilities. These are people that seemingly some anti-research activists would throw under the bus.

I encourage people to be critical thinkers. Do your research and check facts. We live in a democracy where we can robustly debate issues. However, robust debate does not give activists the right to make up alternative facts.

Every academic research university, hospital and non-profit research institution in the Pacific Northwest has joined this fight against COVID-19. Just like the doctors and nurses on the frontlines, the hard work and dedication of these biomedical researchers saves lives and ensures we have a healthier community.

All 175 million of us who are vaccinated in America, and the many who are still protected even though they cannot get vaccinated due to underlying health conditions, should say #ThanksResearch to our local universities and research institutions who are saving lives.

Don’t buy into lies from those who bitterly reject science.

Ken Gordon is executive director of the Northwest Association of Biomedical Research.

This opinion piece also appeared in the Portland Tribune on July 02, 2021

2021 Ignition Awards Recipients Named

2021 Ignition Awards Recipients:

The WaNPRC Pilot Program is conducted jointly with the Institute for Translational Health Sciences and provides funding to collect preliminary data for future funding opportunities. The goal is to fund projects with innovative research endeavors that have translational implications to move toward human applications. This year there was a competitive pool of applications and we were able to provide funding to 2 projects.

Please join us in congratulating the Grant Year 60 recipients of the WaNPRC Pilot Program which exemplify the commitment to cutting edge science, collaboration and also support the 3Rs (Reduction, Refinement and Replacement) of animal use.

  • Jesse Erasmus, PhD,  (University of Washington, Fuller Lab)  “Overcoming bottlenecks in mRNA-mediated antibody expression in nonhuman primates”

Abstract: We have demonstrated protection from acute virus infection in mice receiving an intramuscular injection of RNA encoding a monoclonal antibody. While this approach holds promise for enabling rapid development of antibody therapeutics that can be administered in an outpatient setting, scaling intramuscular doses from mice to larger animals has proven difficult, failing in a nonhuman primate pilot study. We have since identified three major bottlenecks in antibody expression in vivo, 1) host translation shutdown mediated by endoplasmic reticulum (ER) stress and plasmacytoid dendritic (pDC) cell interferon production, 2) anti-drug antibody response, and 3) limiting numbers of transfected target cells. We have developed an RNA molecule that co-expresses a modified antibody along with a cell-autonomous human ICAM-1 blocking peptide, designed to reduce ER stress and inhibit local pDC interaction with RNA-transfected cells, respectively. Given the 87% similarity between human and pigtail macaque (PTM) ICAM-1, we hypothesize that in PTMs, this will 1) reduce ER stress- and pDC-mediated interferon production and downstream host translation shutoff, and 2) reduce pDC-mediated anti-drug antibody responses. In order to increase the number of target cells, we propose to use an FDA-approved multi-needle array in order to spread an injection volume over multiple sites.

  • Amy Orsborn, PhD, (UW Department of Electrical & Computer Engineering and UW Department of Bioengineering, WaNPRC Core Staff): “Developing and validating a new behavioral assay to quantify feedforward and feedback”

Abstract: Our movements are controlled by a combination of predictive feedforward control and reactive feedback control. Incorporating insights from feedforward/feedback control has significantly improved therapies to restore motor function like brain-machine interfaces. However, existing methods to study motor behaviors in non-human primates (NHPs) cannot quantify and disentangle feedforward and feedback control components. This methodological gap limits our ability to study the neural mechanisms of sensorimotor control. Robust control theory methods have been used to directly quantify feedforward and feedback sensorimotor pathways in humans, but these approaches have not yet been tested in NHPs. We propose a study to develop and validate these behavioral assays in NHPs. We will develop assays for both in-cage assays that may accelerate training (aim 1) and laboratory assays for more complex, higher dimensional movements (aim 2). With expertise in both NHP behavioral training and control theory, our team is positioned to rapidly generate critical feasibility data for future grants. If successful, our proposal will provide primate neuroscientists with new tools to study the neural mechanisms of sensorimotor learning and control. Quantifying feedforward and feedback control and their neural signatures will also enable improved brain-machine interface therapies.

We wish the recipients luck in their endeavors and we look forward to hearing about their exciting results next year.

Elizabeth A. Buffalo, PhD | Interim Associate Director for Research


The WaNPRC performs critical biomedical research leading to new advances in science and medicine. WaNPRC researchers are working to develop effective vaccines and therapies for HIV/AIDS and other infectious diseases as well as new advances in genetics, neuroscience, vision, and stem cell biology and therapy. The WaNPRC directly supports the National Institutes of Health’s mission to translate scientific advances into meaningful improvement in healthcare and medicine.

2020 Ignition Awards Recipients Named

2020 Ignition Awards Recipients:

The WaNPRC Pilot Program is conducted jointly with the Institute for Translational Health Sciences and provides funding to collect preliminary data for future funding opportunities. The goal is to fund projects with innovative research endeavors that have translational implications to move toward human applications. This year there was a competitive pool of applications and we were able to provide funding to 2 projects.

Please join us in congratulating the Grant Year 59 recipients of the WaNPRC Pilot Program which exemplify the commitment to cutting edge science, collaboration and also support the 3Rs (Reduction, Refinement and Replacement) of animal use.

Abstract:  Hippocampal theta oscillations occur while attending to incoming stimuli and have been linked to successful memory encoding in humans. This activity is thought to reflect coordinated network processing spanning the entire hippocampus, but the cellular mechanisms supporting the rhythmicity in its microcircuits are unknown in primates. Using acute hippocampal slices, we will electrophysiologically characterize the intrinsic and resonant properties of pyramidal cells to determine how cellular physiology shapes the circuit level behavior. We will also establish how the physiology of principal cells is governed by acetylcholine, which is acutely released during attention to salient stimuli and a known modulator of theta rhythmicity. This project will establish a novel in vitro model of hippocampal microcircuit physiology in primates and will provide a necessary link between physiological studies of murines and humans. The WaNPRC and Tissue Distribution Program (TDP) are uniquely suited to support research using this novel approach, and the experiments of this project lay the foundation for future studies of pathophysiology in the hippocampal network.

  • Gwendolyn Wood, PhD (UW Department of Medicine, Division of Allergy & Infectious Diseases): “Optimizing a model of Mycoplasma genitalium reproductive tract infection in female pig-tailed macaques”

Abstract:  Mycoplasma genitalium (MG) is an emerging reproductive tract pathogen with a disease spectrum similar to N. gonorrhoeae and C. trachomatis, and a prevalence ranging from 1-4% in population-based studies to more than 20% in high risk patients. Of particular concern, MG infection is associated with serious upper reproductive tract sequelae in women. Antimicrobial resistant MG is alarmingly common, with untreatable infections reported in the US. An animal model is needed to understand the mechanisms of pathogenesis of MG and devise alternative treatment and prevention strategies. Building on our previous success with a pigtailed macaque model of lower genital tract infection, we now seek funding to optimize our protocol with the goals of improving infection rates, increasing the duration of infection, observing ascension of MG to upper reproductive tract tissues, and defining the natural history of antibody induction. These studies will help fulfill two mandates set by the MG research community: development of an animal model of PID and the design of a serologic test.

We wish the recipients luck in their endeavors and we look forward to hearing about their exciting results next year.

Elizabeth A. Buffalo, PhD | Interim Associate Director for Research


The WaNPRC performs critical biomedical research leading to new advances in science and medicine. WaNPRC researchers are working to develop effective vaccines and therapies for HIV/AIDS and other infectious diseases as well as new advances in genetics, neuroscience, vision, and stem cell biology and therapy. The WaNPRC directly supports the National Institutes of Health’s mission to translate scientific advances into meaningful improvement in healthcare and medicine.

Conservation Leaders of Tomorrow Look to Yesterday: 20 Years of Community Outreach Education at the Tangkoko Nature Reserve, Indonesia

By Chris Braunger | Communications Manager | WaNPRC


For Dr. Randy Kyes and his colleagues at Sam Ratulangi University (UNSRAT) and the Primate Research Center at Bogor Agricultural University (PSSP-IPB), it is been a year of milestones – two decades in the making.

Dr. Kyes, a professor in the department of Psychology at the University of Washington (UW) and director of the Center for Global Field Study, and head of the Division of Global Programs at the Washington National Primate Research Center (WaNPRC) at the has spent 30 years conducting collaborative field research and education programs around the world. The focus of his international efforts involves promoting the healthy coexistence between humans and wildlife, with particular attention to nonhuman primates. In pursuit of this mission, Kyes, along with his international colleagues, conduct broad-based research projects while providing field courses for local university students and professionals, and conducting outreach education programs for school children K-12.

A shining example of his international collaboration is the long-standing field course and outreach education program at the Tangkoko Nature Reserve in North Sulawesi, Indonesia conducted annually with colleagues from UNSRAT, a local university in the region, and from PSSP-IPB in Bogor, West Java. In May of 2019, they completed their 22nd annual “Field Course in Conservation Biology & Global Health” and their 20th annual outreach education program.

“Our outreach education program and field course go hand in hand. We conducted our first outreach program in Tangkoko back in 2000, a couple years after we had started the annual field course. That first year consisted of a program involving the adults from Batu Putih, a local village that boarders the Reserve. The discussion focused on issues relating to the critically endangered Sulawesi black macaque monkeys (Macaca nigra) in the reserve, e.g., the need for conservation, their role in local ecotourism, and how to manage the increasing conflict such as crop-raiding. We also addressed the unsustainable practice of hunting the monkeys as food/delicacy for special events, a long-standing cultural practice in the region. I remember very clearly one of the village leaders politely thanking us for the program and acknowledging the impact hunting was having on the monkey population. He went on to say that we are “set in our ways’ and it’s is difficult to change. He then offered, “Why don’t you do another outreach program next year for our children, talk with them before they are set in their ways and help them appreciate the need to protect these monkeys?” That next year (2001), during our annual field course, we conducted our outreach education program with the school children from Batu Putih – and we have done so ever since.”

Each year, third graders from two elementary schools in the village attend the outreach education program. The program objectives are to promote environmental awareness, highlight the relationship between environmental health and global health, and generate a sense of commitment to the conservation of biodiversity in the region. The program takes place over half a day and is conducted at their field station in the Reserve. The children walk about 30 minutes from their village to the field station giving them a true sense of the words “field trip.” Field course participants are the “hosts” of the program and are responsible for conducting most of the activities following Kyes and his colleagues’ established format that includes a slide presentation focusing on conservation and global health that highlights local species like the Sulawesi black macaque, an art contest with the theme: promoting good conservation practices, and a small reception for students and teachers including presentation of the art contest awards. The experience of organizing and conducting an outreach education program is intended to give the field course participants the knowledge and confidence to conduct future outreach programs on their own.

More than 900 children have participated in the outreach program since 2001, which is an impressive accomplishment in and of itself. But there is another interesting development that Kyes and his colleagues have been observing for the past several years. It is one they consider to be an even more significant reflection of their efforts. A growing number of the children who were impacted by the early outreach programs have grown up to become participants in the annual Tangkoko field course as young adults.

Kyes discusses the first time this happened: “We were nearing the end of our 13th annual field course in May 2010. We had 10 participants that year including seven university students, one park ranger, and two young women from Batu Putih. I was giving my lecture on community outreach education and providing an overview of the outreach activities we would be conducting at our field station the next day for the local children. As I was concluding the lecture, the two young women from Batu Putih said to me, “We remember the outreach program you did for us when we were in elementary school.” I was stunned… As it turned out, when these two women, Fero and Veronica, were third graders (in 2001), they participated in our very first outreach education program with the local elementary school in Batu Putih. They remembered details of the outreach program, in particular, the conservation art contest. In fact, Veronica received third place in the contest for her drawing of a red-knobbed hornbill. This trend of past outreach students now joining the field course as young adults has been continuing each year.”

To date, 21 young adults from Batu Putih who participated in the early outreach programs have gone on to take the field course. A number of these individuals are now pursuing undergraduate degrees at local universities with plans of returning to Batu Putih to assist with reserve management and ecotourism. Some have even become local conservationists, working with local NGOs and serving as tour guides in Tangkoko. “Knowing the mark this program has left on these young people and how they have continued their commitment to conservation into adulthood reaffirms for me the importance of sustained, long-term outreach education” says Kyes. He also is quick to add that an underlying bases for our work is and always has been that “the success of any conservation or health program depends in part on the ability of the local people to take leading roles in the conduct of those programs.” This is clearly something epitomized by their years of work.

In recent years, additional organizations have begun providing outreach education programs in and around the Tangkoko area. This is something Kyes and his colleagues a very pleased to see. “The collective benefits offered by multiple programs are can have a very positive effect on the long-term conservation of the area’s biodiversity and welfare of the people.” He suggests the results of their recently completed 20-year study (May 2009-2019) monitoring the population density the Sulawesi macaques in the core area of the Tangkoko reserve may be one such indication. Their long-term study has shown some stabilization of the core population from earlier declines. This stabilization may be due in part to an increased appreciation by the local residents (via outreach and education programs) of the importance of conserving the primates. He notes, however, that other areas of the reserve system are still showing reduced numbers of monkeys and that long-term conservation efforts are needed to ensure the sustainability of the population. “Our outreach and field course alumni will undoubtedly play key roles in this effort going forward,” says Kyes.


Footnotes and Acknowledgements:

The outreach education program developed by Kyes and his colleagues in Tangkoko has served as the model that he and his colleagues have used in their other field courses in Indonesia, Thailand, Nepal, China, Bangladesh, Mexico, India, and D.R. Congo.

In recognition of his years of work providing field training and outreach education for local residents (especially at Tangkoko), Kyes has been honored with the International Primatological Society 2004 “Lawrence Jacobsen Education Development Award” – for significant contribution to primate conservation education in Indonesia; and most recently the American Society of Primatologists 2017 “Kyes Award for Excellence in Educational Outreach.”  This was the inaugural awarding of the Kyes Award.

Dr. Kyes would like to show his gratitude to:

Meis Nangoy, Jane Onibala, Indyah Wahyuni, Sylvia Laatung, Moureen Tamasoleng, Hetty Wungouw, Junius Daniel, and Umar Paputungan from the Universitas Sam Ratulangi in Manado, Indonesia (UNSRAT).

Entang Iskandar from Pusat Studi Satwa Primata –  Institut Pertanian Bogor, Indonesia (PSSP-IPB).